[茶碱处理的L-929细胞对干扰素产生的调节作用]
[Effect of modulation of the production of interferon by L-929 cells treated with theophylline].
作者信息
Bokhon'ko A I, Turpaev K T, Itkes A V, Mamontova T V, Orlova T G
出版信息
Mol Gen Mikrobiol Virusol. 1988 Feb(2):28-33.
The specific inhibitor of cAMP phosphodiesterase theophylline has been shown to evoke in L929 cells 2.3-fold induction of 2-5A-synthetase activity and 3.5-fold superinduction of the same enzyme activity while acting in combination with actinomycin D. It has been shown also that temporal coincidence of 2-5A-synthetase induction with the active period of interferon production resulted in 8-16 times decrease in the level of interferon production. The result was supported by the experiments of superinduced cells (containing the high stable level of 2-5A-synthetase) fusion with monolayer of poly(I).poly(C)-induced L929 cells (taken at the start of interferon production). In this case the production of interferon was dramatically decreased in comparison with the control. Possible role of 2-5A-synthetase in regulation of interferon production is discussed.
已证明，环磷酸腺苷磷酸二酯酶的特异性抑制剂茶碱与放线菌素D联合作用时，可使L929细胞中2-5A合成酶活性诱导2.3倍，且使该酶活性超诱导3.5倍。还表明，2-5A合成酶诱导与干扰素产生活跃期的时间重合导致干扰素产生水平下降8至16倍。超诱导细胞（含有高稳定水平的2-5A合成酶）与聚（I）·聚（C）诱导的L929细胞单层（在干扰素产生开始时获取）融合的实验支持了这一结果。在这种情况下，与对照相比，干扰素的产生显著降低。讨论了2-5A合成酶在干扰素产生调节中的可能作用。
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