Narovlianskiĭ A N, Turpaev K T, Amchenkova A M, Itkes A V, Alimov E G
Mol Gen Mikrobiol Virusol. 1988 Feb(2):34-9.
Induction of 2'-5'-oligoadenylatesynthetase (2-5A synthetase) by interferons and theophylline by means of activation of cAMP-system in interferon susceptible and resistant cell lines were studied. In interferon resistant cell lines the basal activity of 2-5A synthetase exceeded the level of the same enzyme in interferon susceptible cell lines. Activity of 2-5A synthetase is increased in interferon susceptible cell lines by interferon treatment, but the activity of the enzyme is not altered in interferon resistant cell lines. Among the studied cell lines the induction of 2-5A synthetase by theophylline was possible only in L929 cell line. The common mechanism for the absence of 2-5A synthetase induction by interferon and theophylline in interferon resistant cells is discussed.
研究了干扰素和茶碱通过激活环磷酸腺苷（cAMP）系统，在对干扰素敏感和耐药的细胞系中诱导2'-5'-寡腺苷酸合成酶（2-5A合成酶）的情况。在干扰素耐药细胞系中，2-5A合成酶的基础活性超过了干扰素敏感细胞系中相同酶的水平。干扰素处理可使干扰素敏感细胞系中2-5A合成酶的活性增加，但在干扰素耐药细胞系中该酶的活性未发生改变。在所研究的细胞系中，只有L929细胞系中茶碱能够诱导2-5A合成酶。讨论了干扰素耐药细胞中干扰素和茶碱无法诱导2-5A合成酶的共同机制。
