Störmer U, Baumgärtel H
Institut für Physikalische Chemie der Freien Universität Berlin, West.
Acta Histochem. 1988;83(1):21-32.
Blood smears and liver and kidney tissues were fixed with different agents, stained with thionine and examined spectroscopically by light microscopy. The obtained absorbance spectra provide results which give information about the influence of the used fixative on the quantity of dye-uptake. Moreover some statements about binding states of the dye in the nuclei of cells can be made. The observed phenomena can be explained by 2 effects: 1. Dissoziation of the DNA-histone-complex, resulting in a demasking of additional binding sites. 2. Extraction of DNA, resulting in a decrease of binding sites. The results are discussed in relation to other relevant parameters concerning fixation and staining.
血液涂片以及肝脏和肾脏组织用不同的试剂固定，用硫堇染色，并通过光学显微镜进行光谱检查。所获得的吸收光谱提供了有关所用固定剂对染料摄取量影响的结果。此外，还可以对染料在细胞核中的结合状态做出一些说明。观察到的现象可以用两种效应来解释：1. DNA-组蛋白复合物的解离，导致额外结合位点的暴露。2. DNA的提取，导致结合位点减少。结合与固定和染色相关的其他参数对结果进行了讨论。
