Marshall P N, Galbraith W, Bacus J W
Anal Quant Cytol. 1979 Nov-Dec;1(3):169-78.
Dye binding to Papanicolaou-stained cervical cells was quantitated with a spectral subtraction technique. It was shown that of the dye components of Papanicolaou stains only aluminum hematein, orange G, light green SF and eosin Y are bound to cervical cells and that their chromophores do not interact. Bismarck brown Y was not bound to cervical cells, which confirms the subjective findings of previous authors, and so it should be omitted. No evidence of substrate-related variations in dye polymerization was found. The proportions of the four dyes were found to vary considerably from substrate to substrate. Spectra are presented of (1) solutions of the component dyes of the Papanicolaou stain, and (2) cervical cells stained with these individual component dyes, for comparison with the full Papanicolaou technique.
采用光谱减法技术对巴氏染色宫颈细胞中的染料结合情况进行了定量分析。结果表明，巴氏染色的染料成分中，只有苏木精铝、橘黄G、亮绿SF和伊红Y能与宫颈细胞结合，且它们的发色团不相互作用。俾斯麦棕Y不与宫颈细胞结合，这证实了先前作者的主观发现，因此应将其省略。未发现与底物相关的染料聚合变化证据。发现这四种染料的比例因底物不同而有很大差异。给出了（1）巴氏染色成分染料溶液和（2）用这些单一成分染料染色的宫颈细胞的光谱，以便与完整的巴氏染色技术进行比较。
