Voronov A V, Sazykin A Iu, Vengerov Iu Iu, Serebriakov M Iu, Malov V A
Antibiot Khimioter. 1988 Jan;33(1):43-6.
A technique for immunoenzymatic diagnosis of dysentery by Shigella sonnei O-antigen was developed. For induction of antibodies to O-antigen rabbits were immunized by intravenous administration of a commercial antidysentery vaccine. Specific antibodies to O-antigen belonging to class G immunoglobulins and not binding to O-antigens of Sh. flexneri and Salmonella typhimurium were obtained. beta-Lactamase of Bacillus licheniformis 749/c was used as a marker enzyme in the immunoenzymatic assay. To increase the sensitivity, beta-lactamase molecules were preliminarily linked with glutaric aldehyde into oligomers. Conjugates of Sh. sonnei O-antigen with the oligomers of B. licheniformis 749/c beta-lactamase were prepared with the periodate method by oxidizing O-antigen. The conjugate was used in competing solid phase immunoenzymatic assay for determination of Sh. sonnei O-antigen in blood serum of patients with dysentery. The sensitivity of the assay is 0.5-1 ng per 1 ml of O-antigen.
开发了一种利用宋内志贺氏菌O抗原进行免疫酶法诊断痢疾的技术。为诱导产生针对O抗原的抗体，通过静脉注射市售抗痢疾疫苗对家兔进行免疫。获得了属于G类免疫球蛋白且不与福氏志贺氏菌和鼠伤寒沙门氏菌的O抗原结合的针对O抗原的特异性抗体。地衣芽孢杆菌749/c的β-内酰胺酶用作免疫酶测定中的标记酶。为提高灵敏度，β-内酰胺酶分子预先与戊二醛连接成低聚物。通过高碘酸盐法氧化O抗原，制备宋内志贺氏菌O抗原与地衣芽孢杆菌749/cβ-内酰胺酶低聚物的结合物。该结合物用于竞争性固相免疫酶测定，以测定痢疾患者血清中的宋内志贺氏菌O抗原。该测定的灵敏度为每1 ml O抗原0.5 - 1 ng。
