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[用于检测宋内志贺菌和福氏志贺菌O抗原的固相检测系统的开发与应用]

[Development and use of solid-phase test-systems for the detection of O-antigen of Shigella sonnei and Shigella flexneri].

作者信息

Triasuchev I O, Davydov A G, Burkin V S, Pokrovskiĭ V I, Pukhova T V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1989 Jun(6):74-7.

PMID:2477970
Abstract

The possibility of the diagnosis of dysentery caused by S. sonnei and S. flexneri, as well as the determination of the dynamics of the distribution of specific O-antigen in the patient's body, by means of the enzyme-linked immunosorbent assay system developed on the basis of antibody preparations obtained by immunosorption has been studied. The study has shown that for better diagnosis the use of fecal extracts is preferable in assays; when used in combination with bacteriological analysis, these assays make it possible to increase the confirmation of the diagnosis of dysentery by several fold.

摘要

通过基于免疫吸附获得的抗体制备开发的酶联免疫吸附测定系统,研究了诊断宋内志贺菌和福氏志贺菌引起的痢疾的可能性,以及确定患者体内特异性O抗原分布动态的方法。研究表明,为了更好地进行诊断,在检测中使用粪便提取物更可取;当与细菌学分析结合使用时,这些检测可以将痢疾诊断的确诊率提高几倍。

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