Manyak M J, Kikukawa T, Mukherjee A B
Experimental Phototherapy Section, National Cancer Institute, Bethesda, MD 20892.
J Urol. 1988 Jul;140(1):176-82. doi: 10.1016/s0022-5347(17)41522-9.
Phospholipase A2 (PLA2) is a key enzyme that initiates the arachidonic acid cascade responsible for the synthesis of prostaglandins and leukotrienes, compounds well known for their inflammatory properties. Inhibition of this enzyme may modulate prostaglandin and leukotriene tissue levels. Uteroglobin is a potent PLA2 inhibitor found in rabbit uterus, prostate, seminal vesicle, and tracheobronchial tree. Tissue from ten human patients undergoing prostatectomy was examined for presence of a uteroglobin-like protein. Seven patients underwent transurethral resection and three had an open prostatectomy. Preoperative diagnosis in nine of the 10 patients was benign prostatic hypertrophy. One suspected, poorly differentiated, adenocarcinoma was confirmed and one unsuspected, well differentiated, adenocarcinoma was discovered. Specimens were submitted for Western blot, electron microscopy with immunogold staining, radioimmunoassay, and immunofluorescence. Six patients had evidence of uteroglobin-like protein, three with high levels (greater than or equal to 1000 pg./mg. protein), two with moderate levels (75 to 250 pg.), one with a low level (less than or equal to 75 pg.). Uteroglobin-like protein was present in all three patients who underwent open prostatectomy and in three of the seven patients with transurethral resections. The uteroglobin-like protein level was 2.5 to five times greater in both prostatic utricle specimens. All four assays corroborated these results. Because rabbit uteroglobin coats sperm and masks spermatic antigenicity in the rabbit female genital tract, this report of biochemical and immunological evidence for uteroglobin-like protein in the human prostate may have implications for human male fertility.
磷脂酶A2（PLA2）是一种关键酶，它启动花生四烯酸级联反应，该反应负责合成前列腺素和白三烯，这些化合物因其炎症特性而广为人知。抑制这种酶可能会调节前列腺素和白三烯的组织水平。子宫珠蛋白是一种强效的PLA2抑制剂，存在于兔子宫、前列腺、精囊和气管支气管树中。对10例接受前列腺切除术的人类患者的组织进行检测，以确定是否存在子宫珠蛋白样蛋白。7例患者接受了经尿道切除术，3例进行了开放性前列腺切除术。10例患者中有9例术前诊断为良性前列腺增生。1例疑似低分化腺癌得到确诊，1例意外发现的高分化腺癌被发现。标本被送去进行蛋白质印迹法、免疫金染色电子显微镜检查、放射免疫测定和免疫荧光检查。6例患者有子宫珠蛋白样蛋白的证据，3例含量高（大于或等于1000 pg./mg蛋白质），2例含量中等（75至250 pg.），1例含量低（小于或等于75 pg.）。子宫珠蛋白样蛋白存在于所有3例接受开放性前列腺切除术的患者以及7例经尿道切除术患者中的3例。在两个前列腺囊标本中，子宫珠蛋白样蛋白水平高2.5至5倍。所有四种检测方法都证实了这些结果。由于兔子宫珠蛋白包裹精子并掩盖兔雌性生殖道中的精子抗原性，因此关于人类前列腺中存在子宫珠蛋白样蛋白的这一生物化学和免疫学证据报告可能对人类男性生育能力有影响。
