Knirel' Iu A, Tanamar N V, Soldatkina M A, Zakharova I Ia
Bioorg Khim. 1988 Dec;14(12):1684-9.
On mild acid degradation of the Pseudomonas cepacia strain IMV 4176 lipopolysaccharide, two polysaccharides were obtained, one of which is a homopolymer of N-acetyl-D-galactosamine and the other is composed of equal amounts of N-acetyl-D-galactosamine and D-ribose. Partial hydrolysis with aqueous oxalic acid caused depolymerization of the heteropolysaccharide, and the homopolysaccharide was isolated in the individual state. On the basis of methylation and 13C NMR analysis, it was concluded that both polysaccharides are built up of disaccharide repeating units having the following structures: ----4)-alpha-D-GalpNAc-(1----4)-beta-D-GalpNAc-(1---- and ----4)-alpha-D-GalpNAc-(1----2)-beta-D-Ribf-(1----. The heteropolysaccharide from P. cepacia strain 4176 is identical by the structure of the repeating unit to the O-specific polysaccharide of P. cepacia strain IMV 4202 (serotype 3), Pseudomonas aeruginosa O12 and Serratia marcescens O14.
洋葱伯克霍尔德菌IMV 4176菌株脂多糖经温和酸降解后，得到两种多糖，其中一种是N - 乙酰 - D - 半乳糖胺的同聚物，另一种由等量的N - 乙酰 - D - 半乳糖胺和D - 核糖组成。用草酸水溶液进行部分水解导致杂多糖解聚，同聚物以单一状态分离出来。基于甲基化和13C NMR分析，得出结论：两种多糖均由具有以下结构的二糖重复单元构成：----4)-α-D-半乳糖胺-(1----4)-β-D-半乳糖胺-(1---- 和 ----4)-α-D-半乳糖胺-(1----2)-β-D-核糖-(1----。洋葱伯克霍尔德菌4176菌株的杂多糖，其重复单元结构与洋葱伯克霍尔德菌IMV 4202菌株（血清型3）、铜绿假单胞菌O12和粘质沙雷氏菌O14的O - 特异性多糖相同。
