Raymond Y, Gagnon G
Institut du Cancer de Montréal, Hôpital Notre-Dame, Québec, Canada.
Biochemistry. 1988 Apr 5;27(7):2590-7. doi: 10.1021/bi00407a048.
Four monoclonal antibodies raised against rat liver nuclear lamins and an anti-intermediate filament antibody [Pruss, R. M., Mirsky, R., & Raff, M. C. (1981) Cell (Cambridge, Mass.) 27, 419-428] have been used to identify epitopes shared by lamin B with lamins A and C, and with intermediate filament proteins. The antibodies defined two major antigenic regions on the three lamins which were both homologous with mouse epidermal keratins as well as hamster vimentin and desmin. Three distinguishable epitopes shared by lamin B with lamins A and C were identified by competition studies between pairs of antibodies and by reaction against N-chlorosuccinimide and cyanogen bromide cleavage fragments. These results support the hypothesis that lamin B, despite important biochemical differences with lamins A and C, shares with them some of the structural characteristics typical of intermediate filament proteins.
针对大鼠肝核纤层蛋白产生的四种单克隆抗体以及一种抗中间丝抗体[普鲁斯，R. M.，米尔斯基，R.，& 拉夫，M. C.（1981年）《细胞》（马萨诸塞州剑桥）27卷，419 - 428页]已被用于鉴定核纤层蛋白B与核纤层蛋白A和C以及中间丝蛋白共有的表位。这些抗体在三种核纤层蛋白上确定了两个主要抗原区域，这两个区域与小鼠表皮角蛋白以及仓鼠波形蛋白和结蛋白均同源。通过抗体对之间的竞争研究以及针对N - 氯代琥珀酰亚胺和溴化氰裂解片段的反应，鉴定出核纤层蛋白B与核纤层蛋白A和C共有的三个可区分表位。这些结果支持这样一种假说，即核纤层蛋白B尽管与核纤层蛋白A和C存在重要的生化差异，但仍与它们共享一些中间丝蛋白典型的结构特征。
