通过菠萝蜜凝集素亲和色谱法从γ-球蛋白制剂中去除包括聚集的IgG在内的不良免疫球蛋白污染物。

Elimination of undesirable immunoglobulin contaminants including aggregated IgG from gamma-globulin preparations by jackfruit lectin affinity chromatography.

作者信息

Kondoh H, Kobayashi K

机构信息

Department of Pediatrics, Yamaguchi University School of Medicine, Japan.

出版信息

Clin Chim Acta. 1988 May 13;174(1):15-23. doi: 10.1016/0009-8981(88)90363-4.

DOI:10.1016/0009-8981(88)90363-4

Abstract

Jackfruit lectin, jacalin, has been characterized to combine with IgA of only IgA1 subclass but not with IgA2 subclass and other immunoglobulins. However, we found in the present study that a lectin from another batch of jackfruit (Jacalin-H) showed a different nature in binding reaction. Jacalin-H combined with immunoglobulins of every class or subclass except monomer IgG. More interestingly, the Jacalin-H also combined with aggregated IgG. By means of the Jacalin-H affinity chromatography, we could efficiently eliminate aggregated IgG as well as immunoglobulins other than monomer IgG from commercial gamma-globulin preparations. Jacalin-H affinity chromatography is easy, inexpensive and efficient in the elimination of undesirable immunoglobulin contaminants in the commercial gamma-globulin preparations.

摘要

波罗蜜凝集素，即jacalin，已被鉴定为仅与IgA1亚类的IgA结合，而不与IgA2亚类及其他免疫球蛋白结合。然而，我们在本研究中发现，另一批波罗蜜中的一种凝集素（Jacalin-H）在结合反应中表现出不同的特性。Jacalin-H能与除单体IgG外的各类或亚类免疫球蛋白结合。更有趣的是，Jacalin-H还能与聚集的IgG结合。通过Jacalin-H亲和层析，我们能够从商业γ球蛋白制剂中有效去除聚集的IgG以及单体IgG以外的免疫球蛋白。Jacalin-H亲和层析在去除商业γ球蛋白制剂中不需要的免疫球蛋白污染物方面简便、廉价且高效。

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Elimination of undesirable immunoglobulin contaminants including aggregated IgG from gamma-globulin preparations by jackfruit lectin affinity chromatography.通过菠萝蜜凝集素亲和色谱法从γ-球蛋白制剂中去除包括聚集的IgG在内的不良免疫球蛋白污染物。

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[Contamination of IgG preparation for intravenous use with IgA. II. Removal of IgA1 from IgG preparation using affinity chomatography on lectin jacalin columns].[静脉用免疫球蛋白制剂被 IgA 污染。II. 使用凝集素红豆蔻素柱亲和层析从免疫球蛋白制剂中去除 IgA1]

Srp Arh Celok Lek. 1994;122 Suppl 1:51-2.

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