Urology Institute, University Hospitals Case Medical Center, and Department of Urology, Case Western Reserve University School of Medicine, Cleveland, OH; Department of Urology, The First Affiliated Hospital, China Medical University, Shenyang, China.
Urology Institute, University Hospitals Case Medical Center, and Department of Urology, Case Western Reserve University School of Medicine, Cleveland, OH.
Urology. 2014 Apr;83(4):958.e1-6. doi: 10.1016/j.urology.2013.12.028. Epub 2014 Feb 16.
To determine if the performance of vaginal distension (VD) in different estrous cycle phases affects the successful induction of stress urinary incontinence in mice.
Female virgin C57BL/6 mice were distributed into 4 groups according to their estrous cycle phase: proestrus, estrus, metestrus, or diestrus. The estrous cycle was staged by examining vaginal smears, and the method was corroborated by histologic examination of the vagina in a subset of each group. Each group was divided into 4 subgroups for measurement of 24-hour micturition behavior and the leak point pressure (LPP) 4 or 20 days after VD or sham VD.
Voiding events increased and mean void volume decreased 4 days, but not 20 days after VD, and the LPP was decreased 4 days, but not 20 days after VD compared with the corresponding sham group, regardless of the estrous cycle phase when VD was performed. There were no differences in 24-hour micturition behavior or LPP among the 4 different estrous cycle phases either in sham or VD group.
Successful induction of reversible stress urinary incontinence in mice is not dependent on the estrous cycle.
确定阴道扩张术（VD）在不同发情周期阶段的表现是否会影响小鼠应激性尿失禁的成功诱导。
根据发情周期阶段，将雌性处女 C57BL/6 小鼠分为 4 组：发情前期、发情期、发情后期或发情间期。通过检查阴道涂片来分期发情周期，并通过对每组的一部分阴道进行组织学检查来证实该方法。每组分为 4 个亚组，在 VD 或假 VD 后 4 或 20 天测量 24 小时排尿行为和漏点压（LPP）。
VD 后 4 天而不是 20 天，排尿事件增加，平均排尿量减少，并且 LPP 降低，而与 VD 进行时的发情周期阶段无关，与相应的假 VD 组相比。无论是在假 VD 组还是 VD 组，4 个不同发情周期阶段的 24 小时排尿行为或 LPP 均无差异。
在小鼠中成功诱导可逆性应激性尿失禁不依赖于发情周期。
