Doble N D, Banga J P, Pope R, Lalor E, Kilduff P, McGregor A M
Department of Medicine, King's College School of Medicine & Dentistry, London, U.K.
Immunology. 1988 May;64(1):23-9.
A detailed examination of the epitopes recognized by autoantibodies (aAbs) to the thyroid microsomal antigen/thyroid peroxidase (TMA/TPO) in patients with thyroid autoimmune disease has been made using a combination of immunochemical and enzymatic techniques. Our results demonstrate the the autoimmune response to thyroid microsomal antigen/thyroid peroxidase (TMA/TPO) is multifocal and far more heterogeneous than hitherto recognized. By immunoblotting with aAbs, antigenic determinants on the TMA/TPO have been recognized that are either susceptible to polypeptide denaturation and/or sensitive to the effects of reducing agents. Furthermore, these aAbs can inhibit, to varying degrees, the enzymatic activity of solubilized preparations of TPO in microsomes, as ascertained by peroxidation of guaiacol and iodide. A large proportion of the autoimmune response is directed to the guaiacol peroxidation site. The data support the view that the autoimmune reactivity to the TMA/TPO is a specific polyclonal response with a minimum of six distinct, independent determinants that are recognized by aAbs.
利用免疫化学和酶学技术相结合的方法，对甲状腺自身免疫性疾病患者中针对甲状腺微粒体抗原/甲状腺过氧化物酶（TMA/TPO）的自身抗体（aAbs）所识别的表位进行了详细研究。我们的结果表明，针对甲状腺微粒体抗原/甲状腺过氧化物酶（TMA/TPO）的自身免疫反应是多灶性的，且比迄今所认识到的更为异质性。通过用自身抗体进行免疫印迹，已识别出TMA/TPO上的抗原决定簇，这些决定簇要么易受多肽变性影响和/或对还原剂的作用敏感。此外，如通过愈创木酚和碘化物的过氧化作用所确定的，这些自身抗体能不同程度地抑制微粒体中TPO可溶性制剂的酶活性。很大一部分自身免疫反应针对愈创木酚过氧化位点。这些数据支持这样一种观点，即对TMA/TPO的自身免疫反应性是一种特异性多克隆反应，至少有六个不同的、独立的决定簇可被自身抗体识别。
