INRA, UR638 Interface Processes and Hygiene of Materials (PIHM), F-59651 Villeneuve d'Ascq, France.
INRA, UR638 Interface Processes and Hygiene of Materials (PIHM), F-59651 Villeneuve d'Ascq, France.
Food Microbiol. 2014 Jun;40:64-74. doi: 10.1016/j.fm.2013.12.004. Epub 2014 Jan 11.
Bacillus strains are often isolated from biofilms in the food industries. Previous works have demonstrated that sporulation could occur in biofilms, suggesting that biofilms would be a significant source of food contamination with spores. In this study, we investigated the properties of mono-species and mixed Bacillus biofilms and the ability of Bacillus strains to sporulate inside biofilms. Bacillus strains were able to form mono-species biofilms on stainless steel coupons, with up to 90% spores after a 48 h-incubation. These spores were highly resistant to cleaning but were easily transferred to agar, mimicking the cross-contamination of food, thereby suggesting that biofilms would be of particular concern due to a potential for Bacillus spore food contamination. This hypothesis was strengthened by the fact that Bacillus strains were able to form mixed biofilms with resident strains and that sporulation still occurred easily in these complex structures.
芽孢杆菌菌株通常从食品工业中的生物膜中分离出来。以前的工作表明，生物膜中可能会发生孢子形成，这表明生物膜将是孢子污染食物的重要来源。在这项研究中，我们研究了单种和混合芽孢杆菌生物膜的特性以及芽孢杆菌菌株在生物膜内形成孢子的能力。芽孢杆菌菌株能够在不锈钢试片上形成单种生物膜，经过 48 小时孵育，最多可产生 90%的孢子。这些孢子对清洁具有很强的抵抗力，但很容易转移到琼脂上，模拟了食物的交叉污染，因此，生物膜可能会引起特别关注，因为存在芽孢杆菌孢子污染食物的潜在风险。这一假设得到了加强，因为芽孢杆菌菌株能够与常驻菌株形成混合生物膜，并且在这些复杂结构中仍然很容易发生孢子形成。
