光照/高温和黑暗/低温培养条件下黏红酵母产脂和类胡萝卜素。
Lipid and carotenoid production by Rhodotorula glutinis under irradiation/high-temperature and dark/low-temperature cultivation.
机构信息
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
出版信息
Bioresour Technol. 2014 Apr;157:149-53. doi: 10.1016/j.biortech.2014.01.039. Epub 2014 Jan 21.
The capacity of lipid and carotenoid production by Rhodotorula glutinis was investigated under different irradiation conditions, temperatures and C/N ratios. The results showed that dark/low-temperature could enhance lipid content, while irradiation/high-temperature increased the yields of biomass and carotenoid. The optimum C/N ratio for production was between 80 and 100. A two-stage cultivation strategy was used for lipid and carotenoid production in a 5L fermenter. In the first stage, the maximum biomass reached 28.1g/L under irradiation/high-temperature. Then, the cultivation condition was changed to dark/low-temperature, and C/N ratio was adjusted to 90. After the second stage, the biomass, lipid content and carotenoid reached 86.2g/L, 26.7% and 4.2mg/L, respectively. More significantly, the yields of biomass and lipid were 43.1% and 11.5%, respectively. Lipids contained 79.7% 18C and 16.8% 16C fatty acids by GC analysis. HPLC quantified the main carotenoids were β-carotene (68.4%), torularhodin (21.5%) and torulene (10.1%).
研究了不同光照条件、温度和 C/N 比下黏红酵母生产脂类和类胡萝卜素的能力。结果表明，黑暗/低温可以提高脂类含量，而光照/高温则增加了生物量和类胡萝卜素的产量。最佳的生产 C/N 比在 80 到 100 之间。采用两阶段培养策略在 5L 发酵罐中生产脂类和类胡萝卜素。在第一阶段，在光照/高温下最大生物量达到 28.1g/L。然后，将培养条件改为黑暗/低温，并将 C/N 比调整到 90。第二阶段后，生物量、脂类含量和类胡萝卜素分别达到 86.2g/L、26.7%和 4.2mg/L。更重要的是，生物量和脂类的产率分别为 43.1%和 11.5%。GC 分析表明，脂质中 79.7%为 18C 脂肪酸，16.8%为 16C 脂肪酸。HPLC 定量分析的主要类胡萝卜素为β-胡萝卜素（68.4%）、番茄红素（21.5%）和 torulene（10.1%）。
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