Kusunoki S, Yu R K, Kim J H
Department of Neurology, Yale University School of Medicine, New Haven, CT 06510.
J Neuroimmunol. 1988 Jul;18(4):303-14. doi: 10.1016/0165-5728(88)90051-3.
Strain 13 guinea pigs were immunized with galactocerebroside, asialo-GM1 (GA1) or GM4 ganglioside in association with myelin basic protein (MBP) in complete Freund's adjuvant (CFA) to produce experimental autoimmune encephalomyelitis (EAE). The clinical and pathological features, serum antibodies, and lipid compositions of affected brains and spinal cords were compared with those of guinea pigs immunized with MBP, in CFA, alone. Perivascular demyelination was seen in brains from all guinea pigs immunized with GA1/MBP. The incidence and degree of demyelination in this group were significantly higher than in the group immunized with only MBP. The onset of EAE was slightly, but significantly, retarded in groups of animals immunized with GM4/MBP and there was no detectable demyelination. Otherwise, no significant differences were detected between groups. Augmentation of EAE by myelin glycolipids may provide some important clues in understanding the mechanism of demyelinating diseases.
将13只豚鼠用半乳糖脑苷脂、脱唾液酸GM1（GA1）或GM4神经节苷脂与髓鞘碱性蛋白（MBP）混合，在完全弗氏佐剂（CFA）中免疫，以诱发实验性自身免疫性脑脊髓炎（EAE）。将受影响的脑和脊髓的临床和病理特征、血清抗体及脂质组成与仅用MBP在CFA中免疫的豚鼠进行比较。在用GA1/MBP免疫的所有豚鼠的脑中均可见血管周围脱髓鞘。该组脱髓鞘的发生率和程度显著高于仅用MBP免疫的组。在用GM4/MBP免疫的动物组中，EAE的发病稍有延迟，但有显著差异，且未检测到脱髓鞘。除此之外，各组之间未检测到显著差异。髓磷脂糖脂对EAE的增强作用可能为理解脱髓鞘疾病的机制提供一些重要线索。
