Wang S, Tang L, Chen F
College of Life Science, Sichuan University, Chengdu 610064, P.R. China, P.R. China.
Plant Cell Rep. 2001 Jul;20(5):393-397. doi: 10.1007/s002990100351. Epub 2014 Feb 15.
Flowers were formed from shoot tips of bitter melon (Momordica charantia L.) cultured on Murashige and Skoog medium supplemented with 90 mM sucrose, 0.05 mM Fe and 4 µM N-benzyladenine (BA). The addition of 0.05 mM Fe to the medium prevented chlorosis of the explant and promoted normal flowering. Increasing the ratio of carbon to nitrogen promoted male flower formation but intensively inhibited vegetative growth. The influence of cytokinin on the morphogenesis of the explant was highly notable. Flowers could be formed after a 15- to 20-day exposure to kinetin (Kin) or BA. Kin and BA had opposite effects with regard to the development of the explant. Kin promoted flower formation, especially female, but inhibited branch bud formation. Conversely, BA promoted branch bud formation and also promoted male flower formation when present at a concentration of 1-2 µM, but completely inhibited flower formation at 4-8 µM. Fluorescein diacetate staining and in vitro germination showed that in vitro pollen were of a fairly high viability.
花是由苦瓜（Momordica charantia L.）的茎尖在添加了90 mM蔗糖、0.05 mM铁和4 μM N-苄基腺嘌呤（BA）的Murashige和Skoog培养基上培养形成的。向培养基中添加0.05 mM铁可防止外植体黄化并促进正常开花。提高碳氮比促进雄花形成，但强烈抑制营养生长。细胞分裂素对外植体形态发生的影响非常显著。在接触激动素（Kin）或BA 15至20天后可形成花。Kin和BA对外植体的发育有相反的影响。Kin促进花的形成，尤其是雌花，但抑制侧芽形成。相反，BA促进侧芽形成，并且当浓度为1-2 μM时也促进雄花形成，但在4-8 μM时完全抑制花的形成。荧光素二乙酸酯染色和体外萌发表明，体外花粉具有相当高的活力。
