Department of Applied Biological Sciences, Saga University, Honjo, 840, Saga, Japan.
Plant Cell Rep. 1996 Mar;15(7):484-8. doi: 10.1007/BF00232979.
Branch internodes of mature plants and stem internodes of seedlings of Fortunella hindsii flowered in vitro on half-strength MT (Murashige and Tucker 1969) basal medium supplemented with benzyladenine, adenine, 6-γ-γ-dimethylallylaminopurine and kinetin. The highest percentage of flowering was achieved with explants originating from branch internodes of flowering plants close to the apex on half-strength MT basal medium containing 5% sucrose and 0.01 mg 1(-1) BA in light. Exposure to darkness for more than 3 weeks followed by re-exposure to light reduced flowering. Flowering required a 4-day exposure to BA, but shoot formation could be initiated even without exposure to BA. First branch internode segments on MT basal medium containing 5% sucrose were prolific in flower (85%) production. The sucrose treatment affected the flower bud size distribution. There were about 13 flower buds per culture in the largest size category (>5 mm).
在含有苄基腺嘌呤、腺嘌呤、6-γ-γ-二甲基烯丙基氨基嘌呤和激动素的半强度 MT（Murashige 和 Tucker，1969）基础培养基上，金橘成熟植株的侧芽和实生苗的茎节离体开花。来自靠近顶端的开花植株侧芽的外植体在半强度 MT 基础培养基（含 5%蔗糖和 0.01 mg 1(-1) BA）上，在光照下，开花率最高。外植体在黑暗中暴露超过 3 周，然后再暴露在光下，会降低开花率。开花需要 4 天的 BA 处理，但即使不接触 BA，也可以启动芽的形成。在含有 5%蔗糖的 MT 基础培养基上的第一侧芽节段大量产生花（85%）。蔗糖处理影响花蕾大小分布。最大大小类别（>5 毫米）中每个培养物约有 13 个花蕾。
