Williamson D J
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
J Histochem Cytochem. 1988 Jul;36(7):811-3. doi: 10.1177/36.7.2454986.
The specificity of the probe for intracellular RNA during hybridization histochemistry is usually confirmed by loss of the signal following ribonuclease pre-treatment of the target. Interpretation of such controls is complicated when single-stranded (asymmetric) RNA probes (riboprobes) are used for in situ hybridization, since the probes themselves may be degraded by residual ribonuclease. A protocol using the Ca2+-dependent enzyme, micrococcal nuclease, is presented to circumvent these difficulties.
在杂交组织化学过程中，细胞内RNA探针的特异性通常通过对靶标进行核糖核酸酶预处理后信号消失来确认。当使用单链（不对称）RNA探针（核糖探针）进行原位杂交时，这种对照的解释会变得复杂，因为探针本身可能会被残留的核糖核酸酶降解。本文提出了一种使用钙依赖性酶微球菌核酸酶的方案来规避这些困难。
