Jeon Jin Young, Kwon Ji-Sue, Kang Soon Tae, Kim Bo-Ra, Jung Yuchul, Han Jae Gap, Park Joon Hyun, Hwang Jae Kwan
Dept. of Biomaterials Science and Engineering, Yonsei University, Seoul, 120-749, Korea; Dept. of Health Food Research & Development of Daesang Corp., Icheon, 467-813, Korea.
Biotechnol Prog. 2014 May-Jun;30(3):736-43. doi: 10.1002/btpr.1889. Epub 2014 Feb 28.
Lutein is a carotenoid with a purported role in protecting eyes from oxidative stress, particularly the high-energy photons of blue light. Statistical optimization was performed to growth media that supports a higher production of lutein by heterotrophically cultivated Chlorella vulgaris. The effect of media composition of C. vulgaris on lutein was examined using fractional factorial design (FFD) and central composite design (CCD). The results indicated that the presence of magnesium sulfate, EDTA-2Na, and trace metal solution significantly affected lutein production. The optimum concentrations for lutein production were found to be 0.34 g/L, 0.06 g/L, and 0.4 mL/L for MgSO4 ·7H2 O, EDTA-2Na, and trace metal solution, respectively. These values were validated using a 5-L jar fermenter. Lutein concentration was increased by almost 80% (139.64 ± 12.88 mg/L to 252.75 ± 12.92 mg/L) after 4 days. Moreover, the lutein concentration was not reduced as the cultivation was scaled up to 25,000 L (260.55 ± 3.23 mg/L) and 240,000 L (263.13 ± 2.72 mg/L). These observations suggest C. vulgaris as a potential lutein source.
叶黄素是一种类胡萝卜素，据称在保护眼睛免受氧化应激方面发挥作用，尤其是蓝光中的高能光子。对支持通过异养培养的普通小球藻提高叶黄素产量的生长培养基进行了统计优化。使用分数析因设计（FFD）和中心复合设计（CCD）研究了普通小球藻的培养基组成对叶黄素的影响。结果表明，硫酸镁、乙二胺四乙酸二钠（EDTA-2Na）和痕量金属溶液的存在显著影响叶黄素的产量。发现硫酸镁·7H2O、EDTA-2Na和痕量金属溶液产生叶黄素的最佳浓度分别为0.34 g/L、0.06 g/L和0.4 mL/L。使用5升罐式发酵罐对这些值进行了验证。4天后，叶黄素浓度几乎增加了80%（从139.64±12.88 mg/L增加到252.75±12.92 mg/L）。此外，当培养规模扩大到25000升（260.55±3.23 mg/L）和240000升（263.13±2.72 mg/L）时，叶黄素浓度并未降低。这些观察结果表明普通小球藻是一种潜在的叶黄素来源。
