University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Bioresour Technol. 2018 Apr;253:141-147. doi: 10.1016/j.biortech.2018.01.027. Epub 2018 Jan 6.
A heterotrophic mutant of Chlorella sorokiniana MB-1-M12 was evaluated for its ability to produce lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal lutein content (3.67 mg lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the lutein production performance. When growing MB-1-M12 strain in a 5L fermenter using the optimal operation strategies, the maximum biomass concentration, biomass productivity, lutein content and lutein productivity could reach 25 g/L, 4.88 mg/L/d, 5.88 mg/g and 16.2 mg/L/d, respectively. This high lutein productivity could significantly reduce the cultivation time and the associated costs, indicating the potential of using MB-1-M12 strain for heterotrophic lutein production in commercial scale.
一株异养型小球藻突变株 MB-1-M12 被评估了利用有机碳源和氮源以及无需光照进行叶黄素生产的能力。在分批发酵中，当在 BG-11 培养基中培养时，可获得最大叶黄素含量（3.67mg 叶黄素/g 生物质）和产率（2.84mg/L/d），其中葡萄糖浓度为 7.5g/L、尿素浓度为 0.75g/L、pH 值为 7.5 和 C/N 比为 10。应用了一种新型的两阶段培养策略，将分批补料和半分批操作相结合，以提高叶黄素的生产性能。当使用最优操作策略在 5L 发酵罐中培养 MB-1-M12 菌株时，最大生物量浓度、生物量产率、叶黄素含量和叶黄素产率分别可达 25g/L、4.88mg/L/d、5.88mg/g 和 16.2mg/L/d。这种高叶黄素产率可以显著缩短培养时间和相关成本，表明 MB-1-M12 菌株在商业规模上用于异养叶黄素生产的潜力。
