Bean J W, Sargent D F, Schwyzer R
Institute of Molecular Biology and Biophysics, Swiss Federal Institute of Technology, Zürich.
J Recept Res. 1988;8(1-4):375-89. doi: 10.3109/10799898809048999.
The heterogeneous environment in which ligand/receptor interactions occur often leads to complex binding behaviour. We consider here the ligand/membrane interaction, emphasizing the possibilities of electrostatic modulation of the overall binding characteristics. The binding of Substance P to neutral or negatively charged planar lipid bilayers was monitored using the capacitance minimization technique. The electrostatic attraction to the charged bilayer potentiates the interaction by more than two orders of magnitude and leads to a nonlinearity in the Scatchard plot of bound vs. bulk concentrations. The Boltzmann accumulation factor, along with the direct measurement of the surface potential, provides an easy explanation of the effect. The general importance of electrostatic accumulation (or repulsion) at surfaces is discussed and the concept applied to examples from the literature.
配体/受体相互作用发生的异质环境常常导致复杂的结合行为。我们在此考虑配体/膜相互作用，着重探讨对整体结合特性进行静电调节的可能性。使用电容最小化技术监测P物质与中性或带负电荷的平面脂质双层的结合。对带电荷双层的静电吸引力使相互作用增强了两个以上数量级，并导致结合浓度与本体浓度的Scatchard图出现非线性。玻尔兹曼积累因子以及表面电位的直接测量为该效应提供了一个简单的解释。讨论了表面静电积累（或排斥）的普遍重要性，并将该概念应用于文献中的实例。
