Stankowski S
Biochim Biophys Acta. 1984 Nov 7;777(2):167-82. doi: 10.1016/0005-2736(84)90418-8.
In previous reports (Stankowski, S. (1983) Biochim. Biophys. Acta 735, 341-351 and 352-360) the ordinary Scatchard-type analysis has been shown to yield erroneous results when applied to the binding of large molecules to membranes or cells. Formulae have been given to treat the limiting cases of very thin and of very bulky ligands. These results are now extended to include ligands of any shape and cooperative interactions. As an example, data on the cooperative binding of polymyxin to charged lipid bilayers are reevaluated. Adsorption with concomitant incorporation of the large molecule into the membrane is also considered.
在之前的报告中（斯坦科夫斯基，S.（1983年）《生物化学与生物物理学报》735卷，341 - 351页以及352 - 360页），普通的斯卡查德型分析已表明，当应用于大分子与膜或细胞的结合时会产生错误结果。已给出公式来处理非常薄和非常庞大配体的极限情况。现在将这些结果扩展到包括任何形状的配体和协同相互作用。例如，对多粘菌素与带电脂质双层协同结合的数据进行了重新评估。还考虑了大分子伴随掺入膜中的吸附情况。
