Wang Wenyu, Han Siwen, Feng Zhuangzhuang, Pei Haixia
School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, China.
Methods Mol Biol. 2025;2916:153-162. doi: 10.1007/978-1-0716-4470-6_14.
Virus-induced gene silencing (VIGS) can be used to validate the functional roles of plant genes by infecting plants with viruses carrying target gene segments. In this chapter, we describe VIGS in rose using tobacco rattle virus (TRV) in RNA virus vectors. By silencing the target genes in rose, the roles of related genes in the process of petal abscission can be studied.
病毒诱导的基因沉默（VIGS）可通过用携带靶基因片段的病毒感染植物来验证植物基因的功能作用。在本章中，我们描述了利用RNA病毒载体中的烟草脆裂病毒（TRV）对玫瑰进行VIGS。通过沉默玫瑰中的靶基因，可以研究相关基因在花瓣脱落过程中的作用。
