Yang Weiyuan, Liu Juanxu, Tan Yinyan, Zhong Shan, Tang Na, Chen Guoju, Yu Yixun
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China,
Plant Cell Rep. 2015 Sep;34(9):1561-8. doi: 10.1007/s00299-015-1808-7. Epub 2015 May 19.
Petunia PhGRL1 suppression accelerated flower senescence and increased the expression of the genes downstream of ethylene signaling, whereas PhGR suppression did not. Ethylene plays an important role in flowers senescence. Homologous proteins Green-Ripe and Reversion to Ethylene sensitivity1 are positive regulators of ethylene responses in tomato and Arabidopsis, respectively. The petunia flower has served as a model for the study of ethylene response during senescence. In this study, petunia PhGR and PhGRL1 expression was analyzed in different organs, throughout floral senescence, and after exogenous ethylene treatment; and the roles of PhGR and PhGRL1 during petunia flower senescence were investigated. PhGRL1 suppression mediated by virus-induced gene silencing accelerated flower senescence and increased ethylene production; however, the suppression of PhGR did not. Taken together, these data suggest that PhGRL1 is involved in negative regulation of flower senescence, possibly via ethylene production inhibition and consequently reduced ethylene signaling activation.
矮牵牛PhGRL1的抑制加速了花朵衰老，并增加了乙烯信号下游基因的表达，而PhGR的抑制则没有。乙烯在花朵衰老中起重要作用。同源蛋白Green-Ripe和乙烯敏感性恢复1分别是番茄和拟南芥中乙烯反应的正调控因子。矮牵牛花朵一直是研究衰老过程中乙烯反应的模型。在本研究中，分析了矮牵牛PhGR和PhGRL1在不同器官、整个花朵衰老过程以及外源乙烯处理后的表达；并研究了PhGR和PhGRL1在矮牵牛花朵衰老过程中的作用。病毒诱导的基因沉默介导的PhGRL1抑制加速了花朵衰老并增加了乙烯生成；然而，PhGR的抑制则没有。综上所述，这些数据表明PhGRL1可能通过抑制乙烯生成从而减少乙烯信号激活，参与了对花朵衰老的负调控。
