School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, Guangdong, China; Institute for Chemical Carcinogenesis, State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510182, Guangdong, China.
Institute for Chemical Carcinogenesis, State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510182, Guangdong, China.
Biomed Environ Sci. 2014 Jan;27(1):10-6. doi: 10.3967/bes2014.011.
To study the alteration of circulating microRNAs in 4-(methylnitrosamino)-1-(3-pyridyl) -1-butanone (NNK)-induced early stage lung carcinogenesis.
A lung cancer model of male F344 rats was induced with systemic NNK and levels of 8 lung cancer-associated miRNAs in whole blood and serum of rats were measured by quantitative RT-PCR of each at weeks 1, 5, 10, and 20 following NNK treatment.
No lung cancer was detected in control group and NNK treatment group at week 20 following NNK treatment. The levels of some circulating miRNAs were significantly higher in NNK treatment group than in control group. The miR-210 was down-regulated and the miR-206 was up-regulated in NNK treatment group. The expression level of circulating miRNAs changed from week 1 to week 20 following NNK treatment.
The expression level of circulating miRNAs is related to NNK-induced early stage lung carcinogenesis in rats and can therefore serve as its potential indicator.
研究 4-（甲基亚硝氨基）-1-（3-吡啶基）-1-丁酮（NNK）诱导的早期肺癌发生过程中循环 microRNAs 的变化。
采用全身给予 NNK 的方法建立雄性 F344 大鼠肺癌模型，应用定量 RT-PCR 法分别于 NNK 处理后 1、5、10 和 20 周检测大鼠全血和血清中 8 种肺癌相关 microRNAs 的水平。
NNK 处理 20 周后对照组和 NNK 处理组均未检出肺癌。NNK 处理组部分循环 microRNAs 的水平明显高于对照组。miR-210 在 NNK 处理组下调，miR-206 在 NNK 处理组上调。NNK 处理后循环 microRNAs 的表达水平从第 1 周变化到第 20 周。
循环 microRNAs 的表达水平与 NNK 诱导的大鼠早期肺癌发生有关，因此可以作为其潜在的标志物。
