Dubey Sonali, Shri Manju, Misra Prashant, Lakhwani Deepika, Bag Sumit Kumar, Asif Mehar H, Trivedi Prabodh Kumar, Tripathi Rudro Deo, Chakrabarty Debasis
Council of Scientific and Industrial Research, National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.
Funct Integr Genomics. 2014 Jun;14(2):401-17. doi: 10.1007/s10142-014-0361-8. Epub 2014 Feb 20.
Industrial growth, ecological disturbances and agricultural practices have contaminated the soil and water with many harmful compounds, including heavy metals. These heavy metals affect growth and development of plants as well as cause severe human health hazards through food chain contamination. In past, studies have been made to identify biochemical and molecular networks associated with heavy metal toxicity and uptake in plants. Studies suggested that most of the physiological and molecular processes affected by different heavy metals are similar to those affected by other abiotic stresses. To identify common and unique responses by different metals, we have studied biochemical and genome-wide modulation in transcriptome of rice (IR-64 cultivar) root after exposure to cadmium (Cd), arsenate [As(V)], lead (Pb) and chromium [Cr(VI)] in hydroponic condition. We observed that root tissue shows variable responses for antioxidant enzyme system for different heavy metals. Genome-wide expression analysis suggests variable number of genes differentially expressed in root in response to As(V), Cd, Pb and Cr(VI) stresses. In addition to unique genes, each heavy metal modulated expression of a large number of common genes. Study also identified cis-acting regions of the promoters which can be determinants for the modulated expression of the genes in response to different heavy metals. Our study advances understanding related to various processes and networks which might be responsible for heavy metal stresses, accumulation and detoxification.
工业增长、生态干扰和农业活动已使土壤和水被包括重金属在内的许多有害化合物污染。这些重金属影响植物的生长和发育，并通过食物链污染对人类健康造成严重危害。过去，人们已开展研究来确定与植物中重金属毒性和吸收相关的生化和分子网络。研究表明，受不同重金属影响的大多数生理和分子过程与受其他非生物胁迫影响的过程相似。为了确定不同金属的共同和独特反应，我们研究了水培条件下，水稻（IR-64品种）根系暴露于镉（Cd）、砷酸盐[As(V)]、铅（Pb）和铬[Cr(VI)]后转录组中的生化和全基因组调控。我们观察到，根组织对不同重金属的抗氧化酶系统表现出不同的反应。全基因组表达分析表明，响应As(V)、Cd、Pb和Cr(VI)胁迫时，根中差异表达的基因数量各不相同。除了独特的基因外，每种重金属还调控了大量共同基因的表达。该研究还确定了启动子的顺式作用区域，这些区域可能是基因响应不同重金属而进行调控表达的决定因素。我们的研究增进了对可能与重金属胁迫、积累和解毒相关的各种过程和网络的理解。
