1Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India.
Am J Alzheimers Dis Other Demen. 2014 Feb;29(1):38-44. doi: 10.1177/1533317513505131.
Low level of vitamin B12 and folic acid has been reported to play an important role in the pathogenesis of Alzheimer's disease (AD) and vascular dementia (VaD). Serum folic acid and vitamin B12 were assayed in 80 AD and 50 VaD cases and in 120 healthy controls. The reduced folate carrier (RFC1) gene, rs1051266, which encodes the RFC 1, protein was analyzed for polymorphism by polymerase chain reaction-restriction fragment length polymorphism. It was observed that the patients having folic acid <8.45 ng/mL had 2.4 (95% confidence interval [CI]: 1.4-4.5) times higher odds of having AD and 2.1 (95% CI: 1.1-4.2) times higher odds of having VaD than patients having folic acid ≥8.45 ng/mL. Serum vitamin B12 level did not show any such statistically significant effect in altering the odds. No direct association was found between variant (G) allele or genotype of rs1051266 with AD and VaD cases. On serum folate level no association was observed with gene polymorphism.
研究表明，维生素 B12 和叶酸水平较低在阿尔茨海默病（AD）和血管性痴呆（VaD）的发病机制中起着重要作用。测定了 80 例 AD 患者、50 例 VaD 患者和 120 名健康对照者的血清叶酸和维生素 B12。通过聚合酶链反应-限制性片段长度多态性分析了编码叶酸载体 1（RFC1）蛋白的还原叶酸载体 1（RFC1）基因 rs1051266 的多态性。结果发现，叶酸<8.45ng/mL 的患者患 AD 的几率是叶酸≥8.45ng/mL 的患者的 2.4 倍（95%可信区间[CI]：1.4-4.5），患 VaD 的几率是叶酸≥8.45ng/mL 的患者的 2.1 倍（95% CI：1.1-4.2）。血清维生素 B12 水平对改变几率没有任何统计学意义。rs1051266 的变异（G）等位基因或基因型与 AD 和 VaD 病例之间没有直接关联。在血清叶酸水平上，未观察到基因多态性与 AD 和 VaD 之间存在关联。
