Kluge M
Botanisches Institut der Technischen Hochschule Darmstadt, Darmstadt, Deutschland.
Planta. 1967 Mar;73(1):50-61. doi: 10.1007/BF00419840.
Sieve tube sap obtained from cucumber plants infected by Cucumis Virus 2 A induced the typical mosaic disease when it was inoculated into healthy plants of the same species. The infectious factor could not be removed by dialysis or by treatment with phosphodiesterase. Therefore it is improbable that the virus is transported in the sieve tubes as low molecular units or as an unprotected RNA. Rod shaped particles (345×23 mμ) were found in the infectious sieve tube sap when it was investigated by electron microscopy. The same particles could be found in the sap extracted from infected leaves, but never in sieve tube sap obtained from healthy plants. There is reason to suppose that the Cucumis Virus 2 A is transported in the sieve tubes as complete particles.
从感染黄瓜花叶病毒2A的黄瓜植株中获取的筛管汁液,接种到同种健康植株上时会引发典型的花叶病。这种感染因子不能通过透析或用磷酸二酯酶处理去除。因此,病毒以低分子单元或无保护的RNA形式在筛管中运输的可能性不大。用电镜研究感染的筛管汁液时,发现了杆状颗粒(345×23微米)。在从感染叶片中提取的汁液中也能发现同样的颗粒,但在从健康植株获取的筛管汁液中从未发现。有理由推测黄瓜花叶病毒2A是以完整颗粒的形式在筛管中运输的。