Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes.
作者信息
Stefanová I, Horejsí V, Kristofová H, Angelisová P, Zizkovský V, Hilgert I
机构信息
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
出版信息
J Immunol Methods. 1988 Jun 28;111(1):67-73. doi: 10.1016/0022-1759(88)90060-9.
Seven murine hybridomas were established producing IgG1 monoclonal antibodies (mAbs) against three different epitopes of human alpha-fetoprotein (alpha FP). The interaction of two of these mAbs (AFP-01 and AFP-02) with the antigen was strongly inhibited by calcium-chelating agents. The mAb AFP-11 could be used for estimation of alpha FP by RIA in the concentration range of 5-100 ng/ml. Several combinations of the mAbs could be used in a two-site sandwich-ELISA for similar purposes. Immunoaffinity chromatography on a column of immobilized mAb AFP-01 permitted the purification of alpha FP.
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