利用杂交瘤培养液通过固相放射免疫分析法检测人甲胎蛋白（AFP）的不同表位
The detection of distinct epitopes of human alpha-fetoprotein (AFP) by solid phase radioimmunoassay using hybridoma culture fluid.
作者信息
Micheel B, Karsten U, Fiebach H, Porstmann T, Denner J
出版信息
Biomed Biochim Acta. 1984;43(11):1317-23.
PMID:6085269
Abstract
A solid phase radioimmunoassay was developed for the epitope analysis of human alpha-fetoprotein (AFP) using hybridoma culture fluids. The following incubation sequence was performed: anti-mouse Ig, hybridoma culture fluid, normal mouse serum, and a mixture of 125I-labeled AFP and hybridoma culture fluid. Seven epitopes were detected by monoclonal antibodies produced by 15 presumably independent hybridoma clones.
摘要
利用杂交瘤培养液开发了一种用于人甲胎蛋白（AFP）表位分析的固相放射免疫测定法。采用了以下温育顺序：抗小鼠Ig、杂交瘤培养液、正常小鼠血清，以及125I标记的AFP与杂交瘤培养液的混合物。通过15个大概独立的杂交瘤克隆产生的单克隆抗体检测到了7个表位。
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