Jiang Jinlin, Wang Xiaorong, Shan Zhengjun, Yang Liuyan, Zhou Junying, Bu Yuanqin
Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Environmental Protection, Nanjing, People's Republic of China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.
PLoS One. 2014 Feb 18;9(2):e88211. doi: 10.1371/journal.pone.0088211. eCollection 2014.
With the rapid development of industry and agriculture and associated pollution, the cyanobacterial blooms in Lake Taihu have become a major threat to aquatic wildlife and human health. In this study, the ecotoxicological effects of cyanobacterial blooms on cage-cultured carp (Cyprinus carpio L.) in Meiliang Bay of Lake Taihu were investigated. Microcystins (MCs), major cyanobacterial toxins, have been detected in carp cultured at different experimental sites of Meiliang Bay. We observed that the accumulation of MCs in carp was closely associated with several environmental factors, including temperature, pH value, and density of cyanobacterial blooms. The proteomic profile of carp liver exposed to cyanobacterial blooms was analyzed using two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The toxic effects of cyanobacterial blooms on carp liver were similar to changes caused by MCs. MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS). MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton. These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins. The results of this study indicated that cyanobacterial blooms pose a major threat to aquatic wildlife in Meiliang Bay in Lake Taihu. These results provided evidence of the molecular mechanisms underlying liver damage in carp exposed to cyanobacterial blooms.
随着工农业的快速发展以及相关污染，太湖蓝藻水华已成为对水生野生动物和人类健康的重大威胁。在本研究中，调查了太湖梅梁湾蓝藻水华对网箱养殖鲤鱼（Cyprinus carpio L.）的生态毒理学影响。微囊藻毒素（MCs）是主要的蓝藻毒素，已在梅梁湾不同实验地点养殖的鲤鱼中检测到。我们观察到鲤鱼体内MCs的积累与几个环境因素密切相关，包括温度、pH值和蓝藻水华密度。使用二维差异凝胶电泳（2D-DIGE）和质谱分析了暴露于蓝藻水华的鲤鱼肝脏的蛋白质组图谱。蓝藻水华对鲤鱼肝脏的毒性作用与MCs引起的变化相似。MCs被转运到肝细胞中并诱导活性氧（ROS）的过量产生。MCs和ROS直接或间接抑制蛋白磷酸酶和醛脱氢酶（ALDH），导致氧化应激和细胞骨架破坏。这些作用通过一系列相关蛋白质的调节进一步干扰了肝脏中的代谢途径。本研究结果表明，蓝藻水华对太湖梅梁湾的水生野生动物构成重大威胁。这些结果为暴露于蓝藻水华的鲤鱼肝脏损伤的分子机制提供了证据。
