Prica Dunja, Tadin Antonija, Marović Danijela, Katunarić Marina, Prica Adriana, Galić Nada
Novi Zagreb Medical Center Zagreb, Croatia.
Department of Endodontics and Restorative Dentistry, Study of Dental Medicine, School of Medicine, University of Split, Split, Croatia.
Acta Clin Croat. 2013 Sep;52(3):309-15.
Dental adhesives come into direct contact with oral tissues. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The aim of this study was to evaluate the genotoxic effect of dental adhesives on human lymphocytes in vitro. Polymerized dental adhesives (Excite, Adper Single Bond 2, Prompt L-pop and OptiBond Solo Plus) were eluted in dimethyl sulfoxide for 1 hour, 24 h and 120 h (5 days). Thereafter, lymphocyte cultures were treated with different concentrations of eluates (0.2 microg/mL, 0.5 microg/mL and 5 microg/mL) obtained from each of the tested materials. Genotoxicity was evaluated by micronucleus test. The chi2-test was used on statistical analysis (p < 0.05). After elution period of 1 h, only the highest dose of all tested materials affected the measured cytogenetic parameters. After 24 h, genotoxicity was demonstrated only in cultures treated with eluates in concentrations of 0.5 microg/mL and 5 microg/mL. Based on the results, it is concluded that the use of dental adhesives causes genotoxic effects in human lymphocytes. Toxic effect of these dental adhesives increases with the tested material concentration and decreases with the length of elution period.
牙科粘合剂会与口腔组织直接接触。由于这种密切且长期的接触，这些材料应具有高度的生物相容性。本研究的目的是在体外评估牙科粘合剂对人淋巴细胞的遗传毒性作用。将聚合后的牙科粘合剂（Excite、Adper Single Bond 2、Prompt L-pop和OptiBond Solo Plus）在二甲基亚砜中洗脱1小时、24小时和120小时（5天）。此后，用从每种测试材料中获得的不同浓度洗脱液（0.2微克/毫升、0.5微克/毫升和5微克/毫升）处理淋巴细胞培养物。通过微核试验评估遗传毒性。采用卡方检验进行统计分析（p < 0.05）。洗脱1小时后，只有所有测试材料的最高剂量影响所测量的细胞遗传学参数。24小时后，仅在用浓度为0.5微克/毫升和5微克/毫升的洗脱液处理的培养物中显示出遗传毒性。根据结果得出结论，使用牙科粘合剂会对人淋巴细胞产生遗传毒性作用。这些牙科粘合剂的毒性作用随测试材料浓度的增加而增加，随洗脱时间的延长而降低。
