1Department of Restorative Dental Medicine and Endodontics, Study of Dental Medicine, School of Medicine, University of Split, Split, Croatia; 2Study of Dental Medicine, School of Medicine, University of Split, Split, Croatia; 3Division for Mutagenesis, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Acta Clin Croat. 2021 Jun;60(2):209-215. doi: 10.20471/acc.2021.60.02.06.
The aim of this cross-sectional study was to investigate the occurrence of chromosomal abnormalities through the frequency of micronuclei and other genomic damage markers in patients with chronic generalized periodontitis and without periodontal disease. Micronucleus assay was performed in exfoliated gingival epithelial cells of 35 patients with generalized chronic periodontitis and 30 control subjects with healthy periodontium. Full mouth clinical examination was performed to define periodontal condition. The mean number of cells with micronuclei observed in chronic periodontitis and control groups was 1.8 (±1.49) and 2.0 (±1.34), respectively. Differences between the groups were not significant (p=0.574). Compared to control subjects, patients with chronic periodontitis showed a significant increase in the number of binucleated cells (p≤0.001) and number of cells with nucleoplasmic bridges (p=0.042). Study results indicated that chronic periodontitis was not associated with higher occurrence of chromosomal damage in gingival cells compared to individuals with healthy periodontium.
本横断面研究旨在通过检测慢性广泛性牙周炎患者和无牙周病患者的微核和其他基因组损伤标志物的频率，来研究染色体异常的发生情况。在 35 名患有广泛性慢性牙周炎的患者和 30 名牙周健康的对照组患者的脱落牙龈上皮细胞中进行了微核检测。进行全口临床检查以确定牙周状况。慢性牙周炎组和对照组观察到的带微核的细胞平均数量分别为 1.8（±1.49）和 2.0（±1.34）。两组之间的差异无统计学意义（p=0.574）。与对照组相比，慢性牙周炎患者双核细胞数量（p≤0.001）和核质桥细胞数量（p=0.042）显著增加。研究结果表明，与牙周健康个体相比，慢性牙周炎患者的牙龈细胞染色体损伤发生率并没有更高。
