Huang Pei-Bei, Chen Shi-Hua, Wang Fei
Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Zhong Yao Cai. 2013 Aug;36(8):1249-52.
To establish the DNA fingerprints of three species from Akebia genus markers and find the feasibility in analyzing their genetic diversity, relationship and germplasm identification by Amplified Fragment Length Polymorphsm (AFLP).
The DNA polymorphism of 12 samples from three species of Akebia genus were detected by AFLP molecular markers.
Among 64 primer pairs,8 were found to yield rich band patterns and high polymorphism. After amplification with these 8 primer pairs to 12 samples, a total of 977 polymorphic DNA bands were detected. The polymorphism rate reached 92.61%. The coefficient of genetic similarity and UPGMA clustering analysis were consistent with the classification based on morphological observations. The coefficient of genetic similarity was 0.49 and the 12 samples could be clustered into 2 groups. Akebia quinata samples formed a single cluster and Akebia trifoliata var. australis and Akebia trifoliata samples formed another cluster.
AFLP markers can be potentially used in analyzing of genetic diversity, relationship and germplasm identification of these medicinal plants.
建立木通属三种植物的DNA指纹图谱标记，并探讨利用扩增片段长度多态性（AFLP）分析其遗传多样性、亲缘关系及种质鉴定的可行性。
采用AFLP分子标记检测木通属三种植物12个样品的DNA多态性。
在64对引物中，筛选出8对扩增条带丰富、多态性高的引物。用这8对引物对12个样品进行扩增，共检测到977条多态性DNA条带，多态性比率达92.61%。遗传相似系数和UPGMA聚类分析结果与形态学分类结果一致，遗传相似系数为0.49，12个样品可聚为2类，五叶木通样品单独聚为一类，三叶木通变种和三叶木通样品聚为另一类。
AFLP标记可用于这些药用植物的遗传多样性分析、亲缘关系研究及种质鉴定。
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