Hadian J, Khadivi-Khub A
Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.
Plant Biol (Stuttg). 2014 Sep;16(5):1020-4. doi: 10.1111/plb.12162. Epub 2014 Feb 18.
The genus Satureja is an important plant with a number of aromatic and medicinal properties. In this research, the relative efficiencies of amplified fragment length polymorphism (AFLP) and selectively amplified microsatellite polymorphic loci (SAMPL) were used to detect genetic relationships among 14 species of Satureja, growing wild in Iran. Eleven AFLP and 14 SAMPL primer combinations produced 999 and 1142 scorable bands, respectively, all of the fragments of which were found to be polymorphic. The average genetic similarity values based on Jaccard's coefficient were 0.24 and 0.21 for AFLP and SAMPL, respectively, indicating considerable distance and diversity in the studied germplasm. The correlation coefficients were statistically significant between both marker systems (r = 0.89). UPGMA derived from the combined binary data matrices of both markers depicted genetic distinctions among the studied species and clustered them into two main clusters and several groups. S. edmondi showed the maximum distance from other species and was placed into a single main cluster, while the maximum similarity was obtained between S. rechingeri and S. khuzistanica. Our results indicate that both marker systems are suitable for differentiating individuals and species of this genus.
鼠尾草属是一种具有多种芳香和药用特性的重要植物。在本研究中，利用扩增片段长度多态性（AFLP）和选择性扩增微卫星多态性位点（SAMPL）的相对效率来检测在伊朗野生生长的14种鼠尾草之间的遗传关系。11个AFLP引物组合和14个SAMPL引物组合分别产生了999条和1142条可计分条带，所有这些片段均为多态性。基于杰卡德系数的平均遗传相似性值，AFLP和SAMPL分别为0.24和0.21，表明在所研究的种质中存在相当大的距离和多样性。两种标记系统之间的相关系数具有统计学意义（r = 0.89）。从两种标记的组合二元数据矩阵得出的UPGMA显示了所研究物种之间的遗传差异，并将它们聚类为两个主要类群和几个组。埃氏鼠尾草与其他物种的距离最大，被归入一个单独的主要类群，而雷氏鼠尾草和胡齐斯坦鼠尾草之间的相似性最大。我们的结果表明，这两种标记系统都适用于区分该属的个体和物种。
