是什么导致犬鼻窦曲霉菌病？一种用于物种鉴定的分子方法。
What causes canine sino-nasal aspergillosis? A molecular approach to species identification.
作者信息
Talbot Jessica J, Johnson Lynelle R, Martin Patricia, Beatty Julia A, Sutton Deanna A, Billen Frédéric, Halliday Catriona L, Gibson Justine S, Kidd Sarah, Steiner Jörg M, Ujvari Beata, Barrs Vanessa R
机构信息
Valentine Charlton Cat Centre, Faculty of Veterinary Science, University of Sydney, Sydney, New South Wales, Australia.
Department of Medicine and Epidemiology, University of California, Davis, CA, USA.
出版信息
Vet J. 2014 Apr;200(1):17-21. doi: 10.1016/j.tvjl.2014.01.009. Epub 2014 Jan 23.
On the basis of phenotypic identification methods, Aspergillus fumigatus is reported as the most commonly identified aetiological agent of canine sino-nasal aspergillosis (SNA). However, definitive identification of Aspergillus spp. using phenotypic features alone is unreliable. The aim of this study was to determine the molecular identities of fungal species causing SNA in dogs. Genomic DNA was extracted from 91 fungal isolates from 90 dogs diagnosed with SNA in Australia, the USA and Belgium, and the ITS1-5.8S-ITS2 ribosomal DNA and partial β-tubulin regions were sequenced. Eighty-eight of 91 (96.7%) isolates were identified as A. fumigatus and 3/91 (3.3%) belonged to Aspergillus section Nigri spp. (Aspergillus tubingensis: 2/91; Aspergillus uvarum: 1/91). These findings confirm that A. fumigatus is the most common aetiological agent of canine SNA. This is the first report to document a pathogenic role for A. tubingensis and A. uvarum in dogs.
基于表型鉴定方法，烟曲霉被报道为犬鼻窦曲霉病（SNA）最常鉴定出的病原体。然而，仅使用表型特征对曲霉属进行明确鉴定是不可靠的。本研究的目的是确定导致犬SNA的真菌种类的分子身份。从澳大利亚、美国和比利时90只被诊断为SNA的犬的91株真菌分离物中提取基因组DNA，并对ITS1-5.8S-ITS2核糖体DNA和部分β-微管蛋白区域进行测序。91株分离物中有88株（96.7%）被鉴定为烟曲霉，3/91（3.3%）属于黑曲霉组（曲霉菌：2/91；乌氏曲霉：1/91）。这些发现证实烟曲霉是犬SNA最常见的病原体。这是首次报道曲霉菌和乌氏曲霉在犬中的致病作用。
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