遗传漂变抑制了空间结构种群中的细菌接合。
Genetic drift suppresses bacterial conjugation in spatially structured populations.
机构信息
FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts.
FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts; Department of Physics, Harvard University, Cambridge, Massachusetts; Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts; Department of Physics and Program in Bioinformatics, Boston University, Boston, Massachusetts.
出版信息
Biophys J. 2014 Feb 18;106(4):944-54. doi: 10.1016/j.bpj.2014.01.012.
Conjugation is the primary mechanism of horizontal gene transfer that spreads antibiotic resistance among bacteria. Although conjugation normally occurs in surface-associated growth (e.g., biofilms), it has been traditionally studied in well-mixed liquid cultures lacking spatial structure, which is known to affect many evolutionary and ecological processes. Here we visualize spatial patterns of gene transfer mediated by F plasmid conjugation in a colony of Escherichia coli growing on solid agar, and we develop a quantitative understanding by spatial extension of traditional mass-action models. We found that spatial structure suppresses conjugation in surface-associated growth because strong genetic drift leads to spatial isolation of donor and recipient cells, restricting conjugation to rare boundaries between donor and recipient strains. These results suggest that ecological strategies, such as enforcement of spatial structure and enhancement of genetic drift, could complement molecular strategies in slowing the spread of antibiotic resistance genes.
conjugation 是水平基因转移的主要机制，它在细菌中传播抗生素耐药性。尽管 conjugation 通常发生在表面相关的生长（例如生物膜）中，但它一直以来都是在缺乏空间结构的充分混合液体培养中进行研究的，而空间结构已知会影响许多进化和生态过程。在这里，我们在固体琼脂上生长的大肠杆菌菌落中可视化了 F 质粒 conjugation 介导的基因转移的空间模式，并通过传统的质量作用模型的空间扩展来定量理解。我们发现，空间结构抑制了表面相关生长中的 conjugation，因为强烈的遗传漂变导致供体和受体细胞的空间隔离，限制了 conjugation 发生在供体和受体菌株之间的罕见边界上。这些结果表明，生态策略，如空间结构的强制和遗传漂变的增强，可能会补充分子策略，以减缓抗生素耐药基因的传播。
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