Kulcsár P, Prestwich G D
Department of Chemistry, State University of New York, Stony Brook 11794-3400.
Anal Biochem. 1988 May 1;170(2):528-31. doi: 10.1016/0003-2697(88)90668-9.
Silver-staining of polyacrylamide gel electrophoresis (PAGE)-separated proteins allows sensitive detection of proteins but severely reduces the ability to detect weak beta-emitters present in the protein band. A simple procedure is described in which silver can be removed from a silver-stained PAGE gel (deargentation) using photographic fixer, and the silver-free gel can be enhanced and used for fluorography. A quantitative study of sensitivity is reported for 3H-labeled bovine serum albumin with a one-dimensional sodium dodecyl sulfate-PAGE slab gel.
对聚丙烯酰胺凝胶电泳（PAGE）分离的蛋白质进行银染可灵敏检测蛋白质，但会严重降低检测蛋白质条带中存在的弱β发射体的能力。本文描述了一种简单方法，其中可使用照相定影剂从银染的PAGE凝胶上去除银（脱银），并且不含银的凝胶可进行增强处理并用于荧光自显影。报道了对一维十二烷基硫酸钠-PAGE平板凝胶上的3H标记牛血清白蛋白的灵敏度定量研究。
