Schiff T A, Freedberg I M
Department of Dermatology, New York University Medical Center, NY 10016.
Electrophoresis. 1989 Jul;10(7):535-6. doi: 10.1002/elps.1150100717.
The Gelcode color-based silver staining system, an improved formulation based on the original publication by Sammons et al. (Electrophoresis 1981, 2, 141-147) has been adapted to automated rapid staining in the PhastSystem Development Unit. The use of elevated temperatures in the fixation, washing, staining, and stabilization steps of the protocol reduces the total time of the process from 18 h to 1 h. The limit of detection, which is at least tenfold more sensitive than the silver staining protocol recommended for the PhastSystem, corresponds to 0.05-0.1 ng of protein per band. The method is applicable to both one- and two-dimensional polyacrylamide gels.
基于Gelcode颜色的银染系统是在Sammons等人（《电泳》，1981年，第2卷，第141 - 147页）原始发表内容基础上改进的配方，已适用于PhastSystem开发单元中的自动快速染色。在该方案的固定、洗涤、染色和稳定步骤中使用高温，将整个过程的总时间从18小时缩短至1小时。检测限比推荐用于PhastSystem的银染方案至少敏感十倍，相当于每条带0.05 - 0.1纳克蛋白质。该方法适用于一维和二维聚丙烯酰胺凝胶。
