微柱阵列上的微图案化
Micropatterning on micropost arrays.
作者信息
Sniadecki Nathan J, Han Sangyoon J, Ting Lucas H, Feghhi Shirin
机构信息
Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA; Department of Bioengineering, University of Washington, Seattle, Washington, USA.
Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.
出版信息
Methods Cell Biol. 2014;121:61-73. doi: 10.1016/B978-0-12-800281-0.00005-1.
Micropatterning of cells can be used in combination with microposts to control cell shape or cell-to-cell interaction while measuring cellular forces. The protocols in this chapter describe how to make SU8 masters for stamps and microposts, how to use soft lithography to replicate these structures in polydimethylsiloxane, and how to functionalize the surface of the microposts for cell attachment.
细胞微图案化可与微柱结合使用，以在测量细胞力的同时控制细胞形状或细胞间相互作用。本章中的方案描述了如何制作用于印章和微柱的SU8母版，如何使用软光刻技术在聚二甲基硅氧烷中复制这些结构，以及如何对微柱表面进行功能化处理以实现细胞附着。
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