Martín-Gómez Laura, Villalba Antonio, Carballal María Jesús, Abollo Elvira
Centro de Investigacións Mariñas, Consellería do Medio Rural e do Mar, Xunta de Galicia, Aptdo 13, 36320 Vilanova de Arousa, Spain.
Centro de Investigacións Mariñas, Consellería do Medio Rural e do Mar, Xunta de Galicia, Aptdo 13, 36320 Vilanova de Arousa, Spain.
Infect Genet Evol. 2014 Apr;23:138-49. doi: 10.1016/j.meegid.2014.02.004. Epub 2014 Feb 18.
Bonamiosis and disseminated neoplasia (DN) are the most important diseases affecting cultured flat oysters Ostrea edulis in Galicia (NW Spain). Previous research using suppresive substraction hybridisation that had been performed addressing the molecular basis of DN as well as the induction and development of the disease in oysters, yielded the whole open reading frame of nine genes: XBP-1, RACK, NDPk, C1qTNF, RPA3, SAP18, p23, ubiquitin and ferritin. These nine genes were characterized in this study. The phylogenetic relationships for each gene were studied using minimum-evolution methods. Quantitative-PCR assays were also developed to analyse the modulation of the expression of these genes by bonamiosis and disseminated neoplasia. Gene expression profiles were studied in haemolymph cells and in various organs (gill, gonad, mantle and digestive gland) of oysters affected by bonamiosis, disseminated neoplasia, both diseases and in non-affected oysters (control). The expression of XBP-1, NDPk, RPA3, SAP18 and ferritin increased in haemolymph cells of oysters with heavy bonamiosis. The expression of C1qTNF; SAP18 and p23 increased in haemolymph cells of oysters with DN. The expression of XBP-1, RACK, NDPk, RPA3 and p23 significantly increased in haemolymph cells of oysters affected by both diseases. There were changes in the expression of a number of genes in different organs depeding on disease stage: RACK expression increased in gills of oysters with bonamiosis, XBP-1 increased in mantle and digestive organs of oysters with light DN and RPA3 expression increased in gonads of oysters with heavy bonamiosis and heavy neoplasia.
博纳米氏病和播散性肿瘤（DN）是影响加利西亚（西班牙西北部）养殖食用牡蛎（Ostrea edulis）的最重要疾病。先前利用抑制性消减杂交技术开展的研究针对DN的分子基础以及牡蛎疾病的诱发和发展展开，获得了九个基因的完整开放阅读框：XBP-1、RACK、NDPk、C1qTNF、RPA3、SAP18、p23、泛素和铁蛋白。本研究对这九个基因进行了表征。使用最小进化法研究了每个基因的系统发育关系。还开发了定量PCR分析方法，以分析博纳米氏病和播散性肿瘤对这些基因表达的调节作用。研究了受博纳米氏病、播散性肿瘤、两种疾病影响的牡蛎以及未受影响的牡蛎（对照）的血淋巴细胞和各个器官（鳃、性腺、外套膜和消化腺）中的基因表达谱。重度博纳米氏病牡蛎血淋巴细胞中XBP-1、NDPk、RPA3、SAP18和铁蛋白的表达增加。DN牡蛎血淋巴细胞中C1qTNF、SAP18和p23的表达增加。受两种疾病影响的牡蛎血淋巴细胞中XBP-1、RACK、NDPk、RPA3和p23的表达显著增加。根据疾病阶段不同器官中一些基因存在表达变化：博纳米氏病牡蛎鳃中RACK表达增加，轻度DN牡蛎外套膜和消化器官中XBP-1增加，重度博纳米氏病和重度肿瘤的牡蛎性腺中RPA3表达增加。
