Institut de Biologie en Santé - IRIS, Groupe d'Etude des Interactions Hôte-Pathogène, Université d'Angers, L'UNAM, Angers, France; Laboratoire de Microbiologie, Ecole Doctorale des Sciences et de Technologie, Université Libanaise, Tripoli, Lebanon.
Laboratoire de Microbiologie, Ecole Doctorale des Sciences et de Technologie, Université Libanaise, Tripoli, Lebanon.
Int J Infect Dis. 2014 Apr;21:21-3. doi: 10.1016/j.ijid.2014.01.004. Epub 2014 Feb 19.
The emergence of carbapenem-resistant Acinetobacter baumannii has been observed worldwide. We describe the first detection of A. baumannii carrying the blaNDM-1 gene in Lebanon, isolated from Syrian patients wounded during the civil war.
Four carbapenem-resistant A. baumannii strains isolated in 2012 in the Tripoli Government Hospital, Lebanon, from civilians wounded during the Syrian war, were analysed. Susceptibility was determined by disk diffusion testing, and resistance to carbapenems was confirmed by Etest. The presence of blaOXA-23-like, blaOXA-24-like, blaOXA-58-like, blaOXA-143-like, and blaNDM was investigated by PCR. Clonal relationships were studied by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and blaOXA-51 sequence-based typing.
All isolates harboured the blaNDM-1 gene and were negative for other tested carbapenemases. They all belonged to the sequence type 85 and formed a single cluster by PFGE. Finally, blaOXA-51-like gene sequencing revealed the presence of the blaOXA-94 variant in all four isolates.
These findings show that Syria constitutes a reservoir for NDM-1-producing bacteria. These results also highlight the need for effective measures to stop the threatening spread of such strains.
耐碳青霉烯鲍曼不动杆菌的出现已在全球范围内被观察到。我们描述了耐碳青霉烯鲍曼不动杆菌携带 blaNDM-1 基因的首次检测结果，该基因是在黎巴嫩的叙利亚战争伤员中分离出来的。
对 2012 年在黎巴嫩的的黎波里政府医院从在叙利亚战争中受伤的平民中分离出的 4 株耐碳青霉烯鲍曼不动杆菌进行分析。通过纸片扩散试验测定药敏性，Etest 法确认对碳青霉烯类的耐药性。通过 PCR 检测 blaOXA-23 样、blaOXA-24 样、blaOXA-58 样、blaOXA-143 样和 blaNDM 的存在。通过脉冲场凝胶电泳（PFGE）、多位点序列分型（MLST）和 blaOXA-51 序列基于分型研究克隆关系。
所有分离株均携带 blaNDM-1 基因，且对其他检测的碳青霉烯酶均为阴性。它们均属于序列型 85，通过 PFGE 形成单一聚类。最后，blaOXA-51 样基因测序显示所有 4 株菌均存在 blaOXA-94 变体。
这些发现表明叙利亚是产 NDM-1 细菌的储存库。这些结果还强调需要采取有效措施阻止此类菌株的威胁性传播。
