Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, Republic of Korea.
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea; Seoul National University Bundang Hospital, Seongnam 463-707, Republic of Korea.
Microbes Infect. 2014 May;16(5):419-28. doi: 10.1016/j.micinf.2014.02.002. Epub 2014 Feb 20.
The newly identified GPI-anchored Plasmodium vivax merozoite surface protein 1 paralog (MSP1P) has a highly antigenic C-terminus that binds erythrocytes. To characterize the antigenicity and immunogenicity of two regions (PvMSP1P-19 and -33) of the highly conserved C-terminus of MSP1P relative to PvMSP1-19, 30 P. vivax malaria-infected patients and two groups of mice (immunized with PvMSP1P-19 or -33) were tested for IgG subclass antibodies against PvMSP1P-19 and -33 antigens. In the patients infected with P. vivax, IgG1 and IgG3 levels were significantly higher than those levels in healthy individuals, and were the predominant response to the two C-terminal fragments of PvMSP1P (p < 0.05). In mice immunized with PvMSP1P-19, IgG1 levels were the highest while IgG2b levels were similar to IgG1 levels. The levels of Th1 cytokines in mice immunized with PvMSP1P-19 or -33 were significantly higher than those in mice immunized with PvMSP1-19 (p < 0.05). Our results indicate that: (i) IgG1 and IgG3 (IgG2b in mice) are predominant IgG subclasses in both patients infected with P. vivax and mice immunized with PvMSP1P-19 or -33; (ii) the C-terminus of MSP1P induces a Th1-cytokine response. This immune profiling study provides evidence that MSP1P may be a potential candidate for vivax vaccine.
新鉴定的 GPI-锚定的间日疟原虫裂殖子表面蛋白 1 同源物（MSP1P）具有高度抗原性的 C 末端，可与红细胞结合。为了表征 MSP1P 高度保守的 C 末端的两个区域（PvMSP1P-19 和 -33）相对于 PvMSP1-19 的抗原性和免疫原性，对 30 名间日疟原虫感染患者和两组小鼠（用 PvMSP1P-19 或 -33 免疫）进行了针对 PvMSP1P-19 和 -33 抗原的 IgG 亚类抗体的检测。在感染间日疟原虫的患者中，IgG1 和 IgG3 水平明显高于健康个体，并且是对 PvMSP1P 的两个 C 末端片段的主要反应（p<0.05）。在用 PvMSP1P-19 免疫的小鼠中，IgG1 水平最高，而 IgG2b 水平与 IgG1 水平相似。用 PvMSP1P-19 或 -33 免疫的小鼠中的 Th1 细胞因子水平明显高于用 PvMSP1-19 免疫的小鼠（p<0.05）。我们的结果表明：（i）IgG1 和 IgG3（小鼠中的 IgG2b）是感染间日疟原虫的患者和用 PvMSP1P-19 或 -33 免疫的小鼠中主要的 IgG 亚类；（ii）MSP1P 的 C 末端诱导 Th1 细胞因子反应。这项免疫分析研究为 MSP1P 可能是间日疟疫苗的潜在候选物提供了证据。
