粒径对颗粒污泥床反应器中自养脱氮性能的影响及微生物机制。

Effect of particle size on the performance of autotrophic nitrogen removal in the granular sludge bed reactor and microbiological mechanisms.

机构信息

Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.

出版信息

Bioresour Technol. 2014 Apr;157:240-6. doi: 10.1016/j.biortech.2014.01.116. Epub 2014 Feb 5.

DOI:10.1016/j.biortech.2014.01.116

Abstract

The effect of particle size on the performance of autotrophic nitrogen removal in the granular sludge bed reactor (GSB-ANR) and microbiological mechanisms were investigated. The results indicated that performance of GSB-ANR process decreased gradually with the increase of the granular sludge size. Indeed small granules ranging between 0.5 and 0.9mm had a higher nitrogen removal capacity than large ones. The reasons of this effect were that (i) the aerobic ammonium oxidizing capacity of microorganisms was the bottle neck of nitrogen removal in GSB-ANR process, and the increase of aerobic ammonium oxidizing activity enhances nitrite production in nitrification and promotes subsequent nitrite consumption during anaerobic ammonia oxidation; (ii) the aerobic/anaerobic zone separation in granular sludge was the key factor affecting the aerobic ammonium oxidizing capacity of microorganisms. The small granules had a larger aerobic functional zone (75.1%) which was profitable for up-regulating the expression level of functional gene in aerobic ammonium oxidizing microorganisms.

摘要

研究了粒径对颗粒污泥床反应器（GSB-ANR）自养脱氮性能的影响及微生物学机制。结果表明，随着颗粒污泥粒径的增大，GSB-ANR 工艺的性能逐渐下降。事实上，粒径在 0.5 到 0.9 毫米之间的小颗粒比大颗粒具有更高的脱氮能力。这种效果的原因是：（i）微生物的好氧氨氧化能力是 GSB-ANR 工艺脱氮的瓶颈，好氧氨氧化活性的增加促进了硝化过程中亚硝态氮的生成，并促进了随后的厌氧氨氧化过程中亚硝态氮的消耗；（ii）颗粒污泥中的好氧/厌氧区分离是影响微生物好氧氨氧化能力的关键因素。小颗粒具有更大的好氧功能区（75.1%），有利于上调好氧氨氧化微生物功能基因的表达水平。