Knox P, Crooks S
Department of Biochemistry, St. George's Hospital Medical School, London, U.K.
J Cell Physiol. 1988 Jun;135(3):467-73. doi: 10.1002/jcp.1041350314.
When human diploid fibroblasts were seeded onto the surface of blood clots, lysis of the clot occurred as a result of the release of cellular plasminogen activator. A number of aspects of this lysis were studied. 1. There was no significant difference in rates of lysis of whole blood clots, platelet-rich plasma clots, and platelet-poor plasma clots brought about by the same number of fibroblasts. 2. Clot lysis was promoted by nondividing cells and by proliferating cells. 3. Using cycloheximide to block protein synthesis it was found that the plasminogen activator released by fibroblasts had an active half-life of less than an hour. 4. When clots were washed prior to the addition of cells then lysis occurred at an increased rate. This was probably due to the removal of alpha 2-antiplasmin from the clots, since when antisera to alpha 2-antiplasmin was added to clots, lysis also proceeded at an increased rate. 5. Medium conditioned by fibroblasts did not promote clot lysis even when antiplasmin was removed by washing or by addition of antisera. 6. Cells had to be in direct contact with the clot in order to bring about lysis; when cells were separated from clots by permeable membranes there was no lysis. 7. When cross-linking of fibrin was reduced by the inhibition of transglutaminase, the rate of clot lysis was increased.
当将人二倍体成纤维细胞接种到血凝块表面时，由于细胞纤溶酶原激活物的释放，血凝块会发生溶解。对这种溶解的多个方面进行了研究。1. 相同数量的成纤维细胞对全血凝块、富含血小板血浆凝块和贫血小板血浆凝块的溶解速率没有显著差异。2. 非分裂细胞和增殖细胞均可促进凝块溶解。3. 使用环己酰亚胺阻断蛋白质合成后发现，成纤维细胞释放的纤溶酶原激活物的活性半衰期不到一小时。4. 在添加细胞之前对凝块进行洗涤，那么溶解速率会加快。这可能是由于从凝块中去除了α2-抗纤溶酶，因为当向凝块中加入α2-抗纤溶酶抗血清时，溶解也会加快。5. 即使通过洗涤或添加抗血清去除了抗纤溶酶，成纤维细胞条件培养基也不会促进凝块溶解。6. 细胞必须与凝块直接接触才能导致溶解；当细胞通过可渗透膜与凝块分离时，不会发生溶解。7. 当通过抑制转谷氨酰胺酶减少纤维蛋白交联时，凝块溶解速率会增加。
