Kawai K, Zweiman B
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia 19104.
J Neuroimmunol. 1988 Aug;19(1-2):159-65. doi: 10.1016/0165-5728(88)90045-8.
To help clarify effector mechanisms in experimental allergic encephalitis (EAE), the cytotoxic effects of myelin basic protein (MBP)-reactive lymphocytes on oligodendrocytes were studied using a 51Cr release assay. MBP-reactive encephalitogenic T cell lines were cytotoxic to 51Cr-labeled oligodendrocyte target cells derived from Lewis rat fetal brain-dissociated culture, when incubated for 6 h in the presence of antigen-presenting cells (APC) and MBP (percent 51Cr release = 65 +/- 3% vs. spontaneous release = 22 +/- 3% vs. normal lymph node cells + APC and MBP = 20 +/- 3%). This reaction is time dependent, likely MHC restricted, and is not just a nonspecific toxic effect against any Lewis target cells since neither fibroblasts nor astrocytes were affected. Other (tetanus toxoid-reactive) lymphoblasts stimulated by specific antigen were not cytotoxic to the oligodendrocytes. These findings suggest that oligodendrocytes might be target cells for MBP-reactive lymphocytes in EAE if antigen presentation is appropriate.
为了帮助阐明实验性变应性脑脊髓炎（EAE）中的效应机制，使用51Cr释放试验研究了髓鞘碱性蛋白（MBP）反应性淋巴细胞对少突胶质细胞的细胞毒性作用。当在抗原呈递细胞（APC）和MBP存在的情况下孵育6小时时，MBP反应性致脑炎性T细胞系对源自Lewis大鼠胎脑解离培养物的51Cr标记的少突胶质细胞靶细胞具有细胞毒性（51Cr释放百分比=65±3%，自发释放=22±3%，正常淋巴结细胞+APC和MBP=20±3%）。这种反应是时间依赖性的，可能受主要组织相容性复合体（MHC）限制，并且不仅仅是对任何Lewis靶细胞的非特异性毒性作用，因为成纤维细胞和星形胶质细胞均未受到影响。由特异性抗原刺激的其他（破伤风类毒素反应性）淋巴母细胞对少突胶质细胞没有细胞毒性。这些发现表明，如果抗原呈递适当，少突胶质细胞可能是EAE中MBP反应性淋巴细胞的靶细胞。
