Kawai K, Heber-Katz E, Zweiman B
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia 19104-6057.
J Neuroimmunol. 1991 Apr;32(1):75-81. doi: 10.1016/0165-5728(91)90074-h.
Cells of a rat/mouse T cell hybridoma reactive to the encephalitogenic peptide of myelin basic protein (MBP) was found to be cytotoxic to 51Cr-labelled rat oligodendrocytes (oligos) inducing 52 +/- 5% 51Cr release vs. 28 +/- 2% spontaneous 51Cr release from replicate oligos. The hybridoma cells were not toxic for rat astrocytes or concanavalin A-stimulated lymphoblasts. Hybridoma T cells reactive to an experimental allergic encephalomyelitis-irrelevant antigen (ovalbumin) were not cytotoxic to oligos. The cytotoxic reaction required cell-cell contact but did not require the in vitro presence of antigen-presenting cells MBP. The target antigen on the oligos is not yet defined. These studies suggest that MBP-reactive T cells can be directly cytotoxic to oligos in the absence of other cell populations.
发现一株对髓鞘碱性蛋白（MBP）致脑炎肽有反应的大鼠/小鼠T细胞杂交瘤细胞，对51Cr标记的大鼠少突胶质细胞（oligos）具有细胞毒性，可诱导51Cr释放52±5%，而来自重复oligos的自发51Cr释放率为28±2%。杂交瘤细胞对大鼠星形胶质细胞或伴刀豆球蛋白A刺激的淋巴母细胞无毒。对实验性变应性脑脊髓炎无关抗原（卵清蛋白）有反应的杂交瘤T细胞对oligos无细胞毒性。细胞毒性反应需要细胞间接触，但不需要体外存在抗原呈递细胞MBP。oligos上的靶抗原尚未确定。这些研究表明，在没有其他细胞群体的情况下，对MBP有反应的T细胞可直接对oligos产生细胞毒性。
