Suzumura A, Bhat S, Eccleston P A, Lisak R P, Silberberg D H
Brain Res. 1984 Dec 24;324(2):379-83. doi: 10.1016/0006-8993(84)90054-4.
Using differential adhesion we successfully isolated relatively pure populations of mouse oligodendrocytes which can be maintained in vitro for more than two months. The highest percentage of galactocerebroside (GalC)-positive oligodendrocytes was 95% at 3 days after isolation. Thereafter, proliferation of astrocytes and fibroblasts occurred more quickly than did oligodendrocyte precursor division. GalC-positive oligodendrocytes rarely incorporate [3H]thymidine so that the use of a mitotic inhibitor (5 X 10(-6)M AraC) reduced the number of non-oligodendrocytes so as to maintain the purity of oligodendrocytes at more than 75% for 14 days in culture. This system will be of use for immunological and virological studies which require viable cultured mouse oligodendrocytes.
利用差异黏附法，我们成功分离出了相对纯净的小鼠少突胶质细胞群体，这些细胞可在体外维持两个多月。分离后3天，半乳糖脑苷脂（GalC）阳性少突胶质细胞的比例最高可达95%。此后，星形胶质细胞和成纤维细胞的增殖比少突胶质细胞前体的分裂更快。GalC阳性少突胶质细胞很少掺入[3H]胸腺嘧啶核苷，因此使用有丝分裂抑制剂（5×10(-6)M阿糖胞苷）可减少非少突胶质细胞的数量，从而在培养14天内将少突胶质细胞的纯度维持在75%以上。该系统将用于需要活的培养小鼠少突胶质细胞的免疫学和病毒学研究。
