Eskhan Asma O, Abu-Lail Nehal I
The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710.
Colloid Polym Sci. 2014 Feb 1;292(2):343-353. doi: 10.1007/s00396-013-3017-7.
A new method to decoupling of bacterial interactions measured by atomic force microscopy (AFM) into specific and nonspecific components is proposed. The new method is based on computing the areas under the approach and retraction curves. To test the efficacy of the new method, AFM was used to probe the repulsion and adhesion energies present between cells cultured at five pH values (5, 6, 7, 8 and 9) and silicon nitride (SiN). Overall adhesion energy was then decoupled into its specific and nonspecific components using the new method as well as using Poisson statistical approach. Poisson statistical method represents the most commonly used approach to decouple bacterial interactions into their components. For all pH conditions investigated, specific energies dominated the adhesion and a transition in adhesion and repulsion energies for cells cultured at pH 7 was observed. When compared, the differences in the specific and nonspecific energies obtained using Poisson analysis and the new method were on average 2.2% and 6.7%, respectively. The relatively close energies obtained using the two approaches demonstrate the efficacy of the new method as an alternative way to decouple adhesion energies into their specific and nonspecific components.
提出了一种将通过原子力显微镜（AFM）测量的细菌相互作用解耦为特定和非特定成分的新方法。该新方法基于计算进针和退针曲线下的面积。为了测试新方法的有效性，使用AFM探测在五个pH值（5、6、7、8和9）下培养的细胞与氮化硅（SiN）之间存在的排斥能和粘附能。然后使用新方法以及泊松统计方法将总粘附能解耦为其特定和非特定成分。泊松统计方法是将细菌相互作用解耦为其成分的最常用方法。对于所有研究的pH条件，特定能量主导粘附，并且观察到在pH 7下培养的细胞的粘附能和排斥能发生了转变。相比之下，使用泊松分析和新方法获得的特定能量和非特定能量的差异平均分别为2.2%和6.7%。使用这两种方法获得的能量相对接近，证明了新方法作为将粘附能解耦为其特定和非特定成分的替代方法的有效性。
