Mafu Akier Assanta, Roy Denis, Goulet Jacques, Magny Pierre
Département de sciences et technologie des aliments, Université Laval, Ste-Foy, Québec, Canada.
Food Research and Development Centre, Agriculture Canada, St-Hyacinthe, Québec, Canada, J2S 8E3. Food Research and Development Centre Contribution Number 177.
J Food Prot. 1990 Sep;53(9):742-746. doi: 10.4315/0362-028X-53.9.742.
This study was carried out to investigate the attachment capabilities of Listeria monocytogenes strain Scott A to stainless steel, glass, polypropylene, and rubber surfaces after short contact times at ambient (20°C) and cold storage temperatures (4°C) using scanning electron microscopy technique. Surface energy value of each surface was estimated by contact angle measurements. All surfaces displayed many possible harborages for L. monocytogenes attachment. Our results indicated that L. monocytogenes cells could attach to all surface types at both temperatures after contact times as short as 20 min or 1 h. Extracellular materials could be observed on the surfaces especially polypropylene and glass incubated at 4 and 20°C for 1 h respectively.
本研究旨在利用扫描电子显微镜技术,研究在环境温度(20°C)和冷藏温度(4°C)下短时间接触后,单核细胞增生李斯特菌斯科特A菌株对不锈钢、玻璃、聚丙烯和橡胶表面的附着能力。通过接触角测量估算每个表面的表面能值。所有表面都显示出许多单核细胞增生李斯特菌可能附着的藏污处。我们的结果表明,在接触20分钟或1小时后,单核细胞增生李斯特菌细胞在这两种温度下都能附着于所有表面类型。在分别于4°C和20°C孵育1小时的表面上,尤其是聚丙烯和玻璃表面,可以观察到细胞外物质。